orgo help, por favor!?

A specific steroid has λmax = 284 nm and molar absorptivity ε = 11,500. What is the concentration of the compound in a solution whose absorbance at 284 nm is A = 0.055 with a sample pathlength of 1.00 cm?

3 Answers

  • You need to use the Beer-Lambert Law:

    A = εcl

    where A is the absorbance at 284 nm, ε is the molar absorptivity, c is the concentration, and l is the pathlength. Solving for c and plugging the values gives:

    c = A/εl = 0.055/[(11,500 M-1cm-1)(1 cm)] = 4.8×10^-6 M or 4.8μM

  • Gina’s just jealous. Go ahead Sam Saun, your answer is completely correct.

  • wrong!!

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