Suppose your professor handed you a test tube with 2.0mL of an E. coli broth in it and told you to make a 10 t?

Suppose your professor handed you a test tube with 2.0mL of an E. coli broth in it and told you to make a 10 to the negative 1 dilution of the entire culture. explain how you would do this and show calculations.

2 Answers

  • A 10^(-1) dilution means to dilute 1 in 10 [or equally, 1:9 (means 1 to 9 or 1 plus 9)].

    That is, take 1 part of broth and add 9 parts of diluent.

    Another way of saying, is take 1 part of broth and make it up to 10 parts with diluent.

    This is the same as taking 1 mL of broth and adding 9 mL of diluent.

    For the whole culture, use the 2.0 mL of broth and add 18.0 mL of diluent.

    Because you are dealing with the ratio, 1:9, you can multiply both or divide both,

    by the same number, and thereby end up with the same ratio.

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