Why is it important not to dilute the initial sample

Why is it important to not dilute an initial sample before it hasbeen loaded onto the chromatography column?

Answer

Main reason that, High concentrated sample to be needed for column chromotography, then we can observe fine separation

(fine layers), if we dilute the sample and loaded on the column leads to uneven separation, sample will be dispersed over the adsorbent and will not form fine layers.


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